Benavides-Piccione, R.; Regalado-Reyes, M.; Fernaud-Espinosa, I.; Kastanauskaite, A.; Tapia-González, S.; León-Espinosa, G.; Rojo, C.; Insausti, R.; Segev, I.; DeFelipe, J. Differential Structure of Hippocampal CA1 Pyramidal Neurons in the Human and Mouse. Cereb. Cortex 2020, 30, 730–752. [ Google Scholar] [ CrossRef] Li, N.; Gittelman, J.X.; Pollak, G.D. Intracellular Recordings Reveal Novel Features of Neurons That Code Interaural Intensity Disparities in the Inferior Colliculus. J. Neurosci. 2010, 30, 14573–14584. [ Google Scholar] [ CrossRef] [ PubMed][ Green Version]

Yang, H.H.; St-Pierre, F. Genetically Encoded Voltage Indicators: Opportunities and Challenges. J. Neurosci. 2016, 36, 9977–9989. [ Google Scholar] [ CrossRef] Xu L, Jiang Z, Mai L, Qing Q. Multiplexed free-standing nanowire transistor bioprobe for intracellular recording: a general fabrication strategy. Nano Lett. 2014;14:3602–7.

Poo, C.; Isaacson, J.S. Odor Representations in Olfactory Cortex: “Sparse” Coding, Global Inhibition, and Oscillations. Neuron 2009, 62, 850–861. [ Google Scholar] [ CrossRef][ Green Version]

Reinhold, K.; Lien, A.D.; Scanziani, M. Distinct recurrent versus afferent dynamics in cortical visual processing. Nat. Neurosci. 2015, 18, 1789–1797. [ Google Scholar] [ CrossRef] [ PubMed]Billet A, Froux L, Hanrahan JW, Becq F. Development of automated patch clamp technique to investigate CFTR chloride channel function. Front Pharmacol. 2017;8:195. Hong G, Viveros RD, Zwang TJ, Yang X, Lieber CM. Tissue-like neural probes for understanding and modulating the brain. Biochemistry. 2018;57:3995–4004. One of the most obvious advantages of using nanoscale electrical devices in intracellular recording is that the small size of the probe can significantly reduce the structural differences between the biomaterials and electronics and keep the homeostasis. New developments in nanofabrication have completed the production of a massive number of designs within the 1–100 nm level, including several one-, two- and three-dimensional nanostructured materials made from silicon, carbon, or a variety of metals [ 1, 39, 40, 41]. Using etching combined with chemical vapor deposition can easily fabricate large amounts of nanowires and nanotubes [ 16]. Through the bottom-up synthesis of functional nanowires on a planar substrate, a vertical and highly parallelized microelectrode array, which enables intracellular recording by directly penetrating the tips of nanowires into the recorded cells or electroporation, can be formed rapidly [ 42]. Moreover, the wide application of optical and electron beam lithography largely increases the fabrication precision, making patterning at the cellular and subcellular length scales routine. A recent study reported a successful attempt to introduce several regions of different doping types in a single nanowire to produce a nanoscale transistor, establishing the basis for nanoscale integrated recording devices [ 13]. Finally, it is practical to functionalize the surface of nanoscale probes with artificial biomaterials such as lipid layers, which would help to reduce the invasiveness in intracellular recording [ 43]. Therefore, nanofabrication technology makes the design of nanoscale noninvasive recording devices more convenient and shows significant potential in developing new intracellular recording systems. Characterization method on the nanoscale Brecht, M.; Sakmann, B. Dynamic representation of whisker deflection by synaptic potentials in spiny stellate and pyramidal cells in the barrels and septa of layer 4 rat somatosensory cortex. J. Physiol. 2002, 543, 49–70. [ Google Scholar] [ CrossRef]

Dittgen, T.; Nimmerjahn, A.; Komai, S.; Licznerski, P.; Waters, J.; Margrie, T.W.; Helmchen, F.; Denk, W.; Brecht, M.; Osten, P. Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proc. Natl. Acad. Sci. USA 2004, 101, 18206–18211. [ Google Scholar] [ CrossRef][ Green Version] Böhm, C.; Peng, Y.; Maier, N.; Winterer, J.; Poulet, J.F.A.; Geiger, J.R.P.; Schmitz, D. Functional Diversity of Subicular Principal Cells during Hippocampal Ripples. J. Neurosci. 2015, 35, 13608–13618. [ Google Scholar] [ CrossRef] [ PubMed][ Green Version] Lenschow, C.; Brecht, M. Barrel Cortex Membrane Potential Dynamics in Social Touch. Neuron 2015, 85, 718–725. [ Google Scholar] [ CrossRef][ Green Version]

Neher, E.; Sakmann, B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 1976, 260, 799–802. [ Google Scholar] [ CrossRef] Poulet, J.F.A.; Fernandez, L.M.J.; Crochet, S.; Petersen, C.C.H. Thalamic control of cortical states. Nat. Neurosci. 2012, 15, 370–372. [ Google Scholar] [ CrossRef]