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Corning® 96-well Clear Flat Bottom Poly-D-Lysine Coated Microplate, 20per Bag, with Lid, Aseptically Manufactured

There was a significant difference in ATP levels between the H and N ( Table 1B and Supplementary Figure) samples stored under the respective conditions as previously observed in bag-based studies ( Yoshida and Shevkoplyas, 2010; D'Alessandro et al., 2020). The presence of this significant difference demonstrated the translatability of this platform for both N and H storage conditions. Overall, based on the two metrics of e-hemolysis and ATP, it can be concluded that the novel storage platform is sufficiently equivalent to the standard bag method for RBC storage for the purpose of initial AS screening and usable under both H and N storage conditions.Corning® 96-well Clear V-Bottom Polystyrene Not Treated Microplate, 25 per Bag, without Lid, Nonsterile We observed that cells cultured in the elastic plate exhibited typical response to cyclic stretch to orient away from the direction of stretch ( Fig 6A). The cellular response to stretch is known to be repeatable because cells constantly sense changes in their mechanical environment and thus return to their original morphology with no preference in orientation upon static culture. This reversible cellular response was observed for over two days, demonstrating the capability of our device allowing for sufficiently long-term cell cultures ( Fig 6B). The present test also confirmed that the bottom of the PDMS membrane was attached to the plate body without leakage of culture media even during the whole process of the stretching. Oest ME, Miller MA, Howard KI, Mann KA. A novel in vitro loading system to produce supraphysiologic oscillatory fluid shear stress. J Biomech. 2014;47: 518–525. pmid:24275439 Electronic supplementary information (ESI) available: Table S1: Relative quantification of N-glycans released from fetuin standard using a 96-well plate sample preparation method and PGC nano-LC-ESI-MS/MS. Table S2: Relative quantification of O-glycans released from fetuin standard using a 96-well plate sample preparation method and PGC nano-LC-ESI-MS/MS. Table S3: N-glycans released from NMuMG cell line using a 96-well plate sample preparation method and PGC nano-LC-ESI-MS/MS. Table S4: Relative quantification of N-glycans released from NMuMG cells using a 96-well plate sample preparation method and PGC nano-LC-ESI-MS/MS. Table S5: Relative quantification of O-glycans released from NMuMG cells using a 96-well plate sample preparation method and PGC nano-LC-ESI-MS/MS. Fig. S1: Elution pattern of N-glycans with and without phosphate on PGC. See DOI: 10.1039/c9mo00180h

Echeverri CJ, Perrimon N. High-throughput RNAi screening in cultured cells: a user’s guide. Nat Rev Genet. 2006;7: 373–384. pmid:16607398 Spruell C, Baker AB. Analysis of a high-throughput cone-and-plate apparatus for the application of defined spatiotemporal flow to cultured cells. Biotechnol Bioeng. 2013;110: 1782–1793. pmid:23280552 Gilbert JA, Weinhold PS, Banes AJ, Link GW, Jones GL. Strain profiles for circular cell culture plates containing flexible surfaces employed to mechanically deform cells in vitro. J Biomech. 1994;27: 1169–1177. pmid:7929466M. Wuhrer, C. A. Koeleman, C. H. Hokke and A. M. Deelder, Rapid Commun. Mass Spectrom., 2006, 20, 1747–1754 CrossRef CAS PubMed. Corning® 96-well Clear Flat Bottom Polystyrene TC-treated Microplates, 10 per bag, with Lid, Sterile Here, we developed the deformable 96-well cell culture plate with the future perspective of coupling with other 96-well-based technologies such as siRNA/shRNA/drug libraries, automated fluorescence plate readers, and micro-channel pipettes. Given its universal design, we expect our system to be utilized, efficiently together with such existing technologies, as a platform allowing for comprehensive analyses of cellular mechanobiological processes.