Paint pen set for Yamaha DRM2 deep red 20 ml + clear varnish 20 ml

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Mirouze M, Reinders J, Bucher E, Nishimura T, Schneeberger K, et al. (2009) Selective epigenetic control of retrotransposition in Arabidopsis. Nature 461: 427–430. He XJ, Hsu YF, Pontes O, Zhu J, Lu J, et al. (2009) NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA–directed DNA methylation. Genes Dev 23: 318–330. Qian, C. et al. Coordinated transcriptional regulation by the UV-B photoreceptor and multiple transcription factors for plant UV-B responses. Mol. Plant 13, 777–792 (2020). A) In vitro methylation assay of DRM2 or C397R on DNA with different sequence contexts. CG, (GAC) 12; CTA, (TAC) 12; CAA, (AAC) 12; CTG, (TGC) 12. Data are means ± SD ( n = 3 replicates). Statistical analysis used two-tailed Student’s t test for the difference from WT: * P< 0.05, *** P< 0.001, and **** P< 0.0001. ( B) Ribbon representation of DRM2 C397R bound to CCG DNA and SAH. Hydrogen-bonding interactions formed between the side chain of R397 and G12 are depicted as dashed lines in expanded view. The bases of C11 and G12 in the expanded view are colored yellow. ( C) Metaplots showing average methylation level of DRM2, C397R, and ddc over TEs for CG, CHG, and CHH contexts. ( D) Representative genomic regions of two TEs on chromosome 3 (AT3TE666360 and AT3TE28430/AT3TE28440) showing the methylation levels of Col-0, ddc, DRM2/ ddc, and C397R/ ddc. ( E) Bar chart showing the total number of DMCs in each context of DRM2/ ddc and C397R/ ddc called against ddc. ( F) Motif of the 4 nucleotides upstream and 5 nucleotides downstream of hyper DMCs in C397R called against ddc ( n = 29,347).Zhong, X. et al. DOMAINS REARRANGED METHYLTRANSFERASE3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis. Proc. Natl Acad. Sci. USA 112, 911–916 (2015).